These assignments are tough. That is why I mentioned in my first post that market analysis and identification of the potential purchaser is such a massive issue in these cases. In the example you gave on 5 vs 10-acre lots, were you breaking it down on a unit price basis? I wasn't 100% sure on your post if you are referring to the total price of a 5-acre lot vs a 10-acre lot being different or a unit price. But at the end of the day, paired data is the most optimal method.
Unless other states do things differently (certainly a possibility), I wouldn't look to eminent domain appraising for your answer. In the example you gave, even if you decide the contributory value of the surplus 1,000 SF is $0, if the value of the whole (assembled tract) is $100,000, that would imply a unit value of $9.09 per square foot. Based on Illinois' interpretation of the unit rule (and I've heard review appraisers say that it is a poor interpretation), if the proposed right-of-way is that specific 1,000 SF in question, the implied compensation is roughly $9,100. That rule can clearly be advantageous to owners in some cases and not representative of the market's reaction to the contribution of that specific strip for your assignment. The above isn't meant as a slight or contradiction to JTip's example, I think he was simply elaborating more on methodology.